Tue - Aug 15, 2017 1:00pm (2 hours)
Learn how to use BLAST as an experimental tool. We will cover the use of filters as BLAST tools and contrast them with the use of PHI-BLAST. We will learn about substitution matrices and how the PSSM relates to PSI-BLAST. We will discuss how to avoid doing a BLAST search and still obtain the relevant information and Blast Variations for structure/function search The Gene Expression Omnibus (GEO) is a public repository that archives and freely distributes microarray, next-generation sequencing, and other forms of high-throughput functional genomic data. Learn how to navigate the GEO interface to retrieve data to inform your experiments. This workshop will acquaint attendees with NCBI blast tool for comparing nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. Attendees will also learn how to query and download experiments that are MIAME-compliant.
Location Countway Library at Harvard Medical School Lower Level 2, Room 025 Countway Library, Lower Level 2, 025