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Analyzing Next generation sequencing Data: Standard data processing and workflow analysis

Wed - Feb 8, 2017 10:00am (3 hours)

The NGS technologies have the potential to dramatically accelerate biomedical research by enabling comprehensive analysis of genomes and transcriptomes to become inexpensive, routine, and widespread tools. This workshop will focus on methods for base‐calling and variant‐calling, for aligning reads to reference sequences (e.g. genomes), and for de novo assembly of short reads into longer sequences. The following tools will be covered on high performance unix computing cluster; quality reports of FASTQ files, trimming and filtering of reads, alignment and coverage objects such as SAM/BAM files using bowtie/bwa, calling SNPs with Samtools, and De novo Assembly using Velvet.

Location Countway Library at Harvard Medical School Lower Level 2, Room 025 Countway Library, Lower Level 2, 025

InstructorGali Reddy, Harvard Catalyst / Countway
Type of ClassBioinformatics - Sequence Tools
Hands-on?Yes
Prerequisites
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